Repeated Cycles of amplification bring about degradation of the probe, resulting in greater fluorescence. TaqMan detection is, therefore, much more particular than dye‐dependent detection, since an increase in fluorescence happens only upon binding of your probe to a specific sequence and subsequent de‐quenching, even though no sign is produced by https://lingjunbio.quora.com/Application-of-Magnetic-Beads-https-www-lingjunbio-com-products-magnetic-beads-Nucleic-acid-extraction-used-for-rap